The CRISPR-Cas immune system: Biology, mechanisms and applications

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The CRISPR-Cas immune system: biology, mechanisms and applications.

Viruses are a common threat to cellular life, not the least to bacteria and archaea who constitute the majority of life on Earth. Consequently, a variety of mechanisms to resist virus infection has evolved. A recent discovery is the adaptive immune system in prokaryotes, a type of system previously thought to be present only in vertebrates. The system, called CRISPR-Cas, provide sequence-specif...

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Prokaryotes have evolved several defence mechanisms to protect themselves from viral predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated proteins (Cas) display a prokaryotic adaptive immune system that memorizes previous infections by integrating short sequences of invading genomes-termed spacers-into the CRISPR locus. The spacers interspaced with ...

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CRISPR-Cas: the effective immune systems in the prokaryotes

Approximately all sequenced archaeal and half of eubacterial genomes have some sort of adaptive immune system, which enables them to target and cleave invading foreign genetic elements by an RNAi-like pathway. CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems consist of the CRISPR loci with multiple copies of a short repeat sequence separa...

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The discovery of biological concepts can often provide a framework for the development of novel molecular tools, which can help us to further understand and manipulate life. One recent example is the elucidation of the prokaryotic adaptive immune system, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) that protects bacteria and archaea against viruses ...

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Amlinger, L. 2017. The type I-E CRISPR-Cas system. Biology and applications of an adaptive immune system in bacteria. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1466. 61 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9787-3. CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered reg...

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ژورنال

عنوان ژورنال: Biochimie

سال: 2015

ISSN: 0300-9084

DOI: 10.1016/j.biochi.2015.03.025